http://repository.uhamka.ac.id/882/ Prof. Dr. Atmazaki, Atmazaki http://repository.uhamka.ac.id/882/1/B15.pdf Download Disini !

http://repository.uhamka.ac.id/881/ Prof. Dr. Atmazaki, Atmazaki http://repository.uhamka.ac.id/881/1/B14.pdf Download Disini !

http://repository.uhamka.ac.id/879/ Prof. Dr. Atmazaki, Atmazaki http://repository.uhamka.ac.id/879/1/B13.pdf Download Disini !

Berdasarkan data dari Riset Kesehatan Dasar (Riskesdas) 2013 kelompok remaja hingga dewasa muda (15-24) memiliki gaya hidup tidak aktif (sedentary/kurang beraktivitas fisik) sebesar 52%. Kurangnya aktivitas fisik dapat menyebabkan risiko kesehatan terutama timbulnya komplikasi penyakit tidak menular seperti obesitas, diabetes, hipertensi dan gangguan jantung. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fisik dan determinannya. Penelitian ini menggunakan metode kuantitatif dengan desain studi potong lintang. Sejumlah 846 mahasiswa Fikes Uhamka berpartisipasi dalam penelitian ini. Data dikumpulkan dengan mengisi sendiri kuesioner oleh mahasiswa. Analisis data menggunakan uji chi square untuk mengetahui hubungan antara aktifitas fisik dengan determinannya dan regresi logistic untuk mengetahui prediktor dari aktifitas fisik. Penelitian ini memberikan hasil bahwa rata-rata skor Metabolic Equivalent (MET) adalah 1420,33 ± 2384,297. Responden yang memiliki aktivitas fisik yang rendah sebesar 47,8%, aktivitas fisik sedang sebesar 39,6% dan aktivitas fisik tinggi hanya 12,6% responden. Ada hubungan yang bermakna antara jenis kelamin, status pekerjaan, ketersediaan fasilitas olahraga, dukungan dosen, dan persepsi terhadap olahraga terhadap aktivitas fisik. Aktivitas fisik aktif lebih banyak ditemui pada responden kelamin laki-laki, mahasiswa yang sudah bekerja, ketersediaan sarana olahraga di rumah atau lingkungan sekitar, adanya ajakan dosen untuk berolahraga dan adanya persepsi yang baik terhadap olahraga. Hasil penelitian juga menunjukkan bahwa responden yang tidak bekerja, tidak terdapat fasilitas olahraga di rumah, dan mempunyai persepsi yang kurang baik terhadap olahraga adalah prediktor untuk memiliki aktivitas fisik pasif/ringan.

Lactic Acid Bacteria (BAL) are often found naturally in food ingredients such as vegetables and fruits. Cabbage fermentation is one of the best sources of Lactic Acid Bacteria which contain antibacterial compounds such as bacteriocin, hydrogen peroxide, and organic acids. This study purpose are to isolate BAL, screen the antibacterial activity, and identification of molecular of selected isolates. This study was initiated with Lactic Acid Bacteria isolation from cabbage fermentation, followed by screening for antibacterial activity by disc diffussion method and identification of molecular isolates which having the highest antibacterial activity by PCR method. After isolation, 6 isolates were obtained: K31, K32, K33, K34, K35 and K36. The result of antibacterial activity test showed that K32 isolate had the highest activity against bacterium of Bacillus cereus. Molecular identification with PCR method and sequencing of amplification results showed that K32 isolates having 99% similarity level to lactobacillus buchneri JCM 115 strain. From the results of the study it can be concluded that cabbage fermentation contains Lactic Acid Bacteria which have antibacterial activity against Bacillus cereus.

Trees and shrubs of the genus Cordia are widely distributed in the warmer regions, including Indonesia. The aim of this study was to evaluate the analgesic and anti-inflammatory properties of the ethanolic extract of plant leaves in Wistar albino rats. The analgesic activity was evaluated using the hot plate method and acetic acid-induced writhing, and the anti-inflammatory activity was determined using carrageenan-induced paw oedema. The results showed that the Cordia sebestena ethanol extract (100, 200 and 400 mg/kg) exhibited significant analgesic effects in a dose-dependent manner in the two pain models tested. The extract also exhibited significant anti-inflammatory effects in the carrageenan-induced inflammation test. The data obtained support the traditional folklore therapeutic claim about its analgesic and anti-inflammatory properties. Nonetheless, further scientific investigation is required to establish its analgesic and anti-inflammatory properties in other experimental models and clinical settings.

Green tea leaf (Camellia sinensis L.) is a great plant containing polyphenol compounds such as catechin and gallocatechin gallate that can be used as tyrosinase inhibitors. In this study, the green tea leaf extract made into gel dosage form using sodium alginate as a gelling agent. This research aimed to determine the effect of different sodium alginate concentrations on the physical stability of gel extract green leaf and to know the effect of gel formulation on tyrosinase inhibition. Gel made into 3 formulas with each concentration of sodium alginate of 2; 2.5; 3%. The extraction of green tea leaves was performed by reflux using 50% methanol solvent. The results showed that the three formulas did not occur in phase separation, and had IC50 values of 34.80, 23.27, 20.69 μg/mL. The conclusion is gel dosage form with 3% sodium alginate gave the best inhibition of tyrosinase with IC50 of 20.69 μg/mL.

Glutathione (GSH) is a broad antioxidant of the thiol-tripeptide group, highly hydrophilic, which has limitation for topical preparations. A lipophilic surfactant is an alternative method to enhance the glutathione partition. The purpose of this study was to determine the apparent partition coefficient (APC log) of glutathione; glutathione with additional surfactant at different HLB value of HLB 4.3; 5.5; 7; 11 and selected HLB was studied for penetration. The study was conducted by dissolving glutathione in water plus various HLB surfactants. Determination method of partition coefficient was done by shake flash method. The penetration test was conducted using the parameter of decreasing Matrix Metalloproteinase-1 expression on the balb-c male skin. The results can be used as a reference for topical glutathione formulations as these results are preformulation study.

Background: The present study aimed to formulate and evaluate the stability, characteristics and effectiveness of glutathione-loaded alginate microspheres through increased lipophilicity using surfactant with a Hydrophylic-Lipophilic Balance (HLB) value equal to 7. The selection of glutathione as an antioxidant was based on its prominent role in maintaining intracellular redox balance. Alginate was used as the polymer, while calcium chloride constituted a cross-linking agent and Tween and Span were employed as surfactants. Methods: The study applied an ionotropic gelation-aerosolization method. Microspheres were characterized by their morphology, size, drug loading, entrapment efficiency and yield. Stress testing utilized a forced degradation method, while an effectiveness study of glutathione incorporated a Matrix Metalloproteinase I (MMP-1) parameter on mouse skin. Glutathione-microspheres, to which had been added surfactants with a HLB value equal to 7, were compared to those without surfactants. Results: Microspheres demonstrated both high yield and encapsulation efficiency. From the stability study conducted, it was evident that the glutathione-microspheres with additional surfactant were more stable than glutathione with surfactant, but without microspheres. Similarly, the glutathione-microspheres with additional surfactant were more stable than the glutathione without surfactant. The in vivo effectivity showed lipophilic glutathione microspheres were able to decrease MMP-1 expression in the dermis tissue of mice. Conclusion: The results of freeze-dried glutathione-loaded alginate microspheres with surfactant with a HLB value equal to 7 can be utilized as potential glutathione delivery systems.

Indonesian people use bay leaves as spices in local culinary and as traditional medicine, particularly to treat diabetes. The problems with the mass production of antidiabetic drugs from bay leaves can be solved by utilizing endophytic fungi as an alternative source for antidiabetic compounds. This study aimed to isolate endophytic fungi from bay leaves and identify their antidiabetic activity through the in vitro inhibition of alpha-amylase. The leaves were processed on potato dextrose agar media, and five isolates were grown in an agar medium. The fermentation used a potato dextrose yeast medium that was left for five days on an orbital shaker at room temperature. The crude was extracted using ethyl acetate solvent. In the in vitro alpha-amylase inhibition test, the antidiabetic assay used the ethyl acetate extract of the endophytic fungi. The inhibition percentage was calculated from the absorbance value read by a microplate reader. All isolates inhibited alpha-amylase activity, but only three of them had high inhibition percentages (14.385%, 12.849%, and 39.246%). As a conclusion, the endophytic fungi isolated from bay leaves are potential as an alternative source for the production of secondary metabolites to cure diabetes.