Theatrical activities have been used to promote an authentic learning environment. However, the incorporation of simple daily technology, like smartphones, to enable students to produce a video of their theatrical activities has not been explored in the Indonesian language learning classroom. This paper documents a video project conducted by lower secondary school students at a private school in Jakarta, Indonesia to examine whether a video project task could help create an authentic learning environment in a language classroom setting. Although the benefits of theatrical activities to promote authenticity in students’ learning experiences are evident in the literature, only a few students have access to such benefits. Many students were reluctant to get involved in the theatrical activities prepared by the teachers and only one cohort of students successfully completed the advertisement video assignment. Teachers’ instructional issues as well as students’ motivation and commitment to work collaboratively were identified as the barriers to produce the video.

This article aims to describe the intertextuality contained in the legend of Malin Kundang (MK) with the drama script of Malin Kundang Sues (MKS) by Sinar Hadi. The method used is content analysis to describe data objectively and systematically. As a result, expansion is an improvement or development of a hypogram, whereas conversion is a reversal of the hypogram or matrix. In conclusion, the shift in cultural background occurred in Malin Kundang's legend.

Turmeric is one of the plants that can be used as traditional medicine. To improve the quality of turmeric as a traditional medicine, turmeric must be free from contamination of pesticide residues, aflatoxin, pathogen bacteria, and curcumin content contained therein. The aim of this research was to investigate the contamination of endosulfan and malathion pesticides, aflatoxin B1, Escherichia coli microbial contamination, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa, as well as to know the content of curcumin contained in turmeric rhizomes. The sample in this research was taken from Wonogiri region of Central Java, Indonesia by random sampling. The methods used were HPLC for Aflatoxin B1 analysis and curcumin and Gas Chromatography for residual pesticide analysis of Endosulfan and Malathion pesticides. Microbial testing included the establishment of Total Plate Count, AKK, MPN Coliform, and analysis of Escherichia coli microbial contamination, Salmonella sp., Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that the samples were not contaminated by Aflatoxin B1 and Endosulfan pesticides, but contained a residual malathion with levels of 0.014 mg/kg. Microbial test results showed that the turmeric samples from the Wonogiri market did not meet the quality requirements due to contamination of Salmonella sp. and the chopped AKK exceeded the specified limits.

Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic bacteria producing secondary metabolites. Several studies have reported that secondary metabolite compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion method and identification of molecular isolates which had the highest antibacterial activity. After isolation, three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which have antibacterial activity against Bacillus subtilis and Salmonella typhi.

Menurut data SUDINKES DKI Jakarta tahun 2016 menunjukkan bahwa kotamadya Jakarta Timur memiliki jumlah populasi yang hipertensi lebih banyak dibandingkan dengan daerah lainnnya yaitu sebanyak 98422 orang. Konsumsi buah dan sayuran segar akan memberikan banyak manfaat bagi tubuh kita, bukan saja rasanya yang enak tapi buah dan sayur kaya akan antioksidan yang bermanfaat untuk mencegah terjadinya stres oksidatif dan bisa menjadi salah satu upaya dalam mencegah dan mengobati hipertensi. Berbagai buah dan sayuran banyak yang telah diteliti memberikan khasiat antihipertensi. Pada pengabdian masyarakat ini kami mengadakan Pelatihan pembuatan nutrisi dan penyuluhan penyakit hipertensi pada anggota PKK RT. 014 Perumnas Klender Jakarta Timur. Buah-buahan dan sayur-sayuran yang kami gunakan adalah belimbing, semangka, lemon, seledri dan mentimun.

Papaya seed in previous research is known has an effect of anthelmintic on Ascardia galli, but the effect of papaya seed just by traditional method i.e. boiling in water. To get the effective extraction methods, it still needs to be done many kinds of extraction methods of papaya seed, to know which one is the best. Methods of extraction in this research were maceration, soxhletation, and ultrasonic digestion. We used two kinds of papaya seed: wet and dry, using solvent: water, 70% ethanol, and n-hexane. Data of anthelmintic activity were percentage of worm death, LD50, and relative potential to pyrantel pamoate as positive control. The result showed that the best extraction method of papaya seed which had highest in vitro effect on Ascardia galli worm was maceration method with 70% ethanol as solvent of wet papaja seed. It killed 60% of worm with LD50 9.36 mg and its relative potential is 0.66 times pyrantel pamoate.

Ekstrak buah mengkudu (Morinda Citrifolia L.) memilki banyak kandungan kimia salah satunya Scopoletin. Namun perbedaan letak geografis suatu tanaman dapat mengakibatkan terjadinya variasi kandungan metabolit dari suatu tanaman. Tujuan penelitian ini untuk mengetahui parameter fisikokimia pada ekstrak etanol 70% buah mengkudu (Morinda Citrifolia L.) dan untuk mengetahui apakah perbedaan daerah tumbuh dapat berpengaruh pada kadar scopoletin pada ekstrak etanol 70% buah mengkudu. Hasil pengujian didapat ekstrak yang berasal dari purwakarta, sari larut air 26,0793 % , sari larut etanol 11,6825 %, kadar abu 1,6517%, kadar abu tidak larut asam 0,3075%, kadar air 11,3014% sedangkan yang berasal dari bogor, sari larut air 42,4098%, sari larut etanol 22,2071%, kadar abu 1,0536%, kadar abu tidak larut asam 0,1001%, kadar air 8,0825%.Pengujian Kadar scopoletin dilakukan dengan metode KLT-Densitometri. KLT menggunakan Fase diam silika gel GF254 dan fase gerak eter: toluen: asam asetat 10% (58:45:0,8). Kadar scopoletin rata-rata daerah Purwakarta adalah 1,4895% dan untuk daerah Bogor adalah 2,4505%. Berdasarkan hasil penelitian mutu ekstrak yang lebih baik adalah buah mengkudu yang berasal dari daerah Bogor dengan nilai parameter non spesifik lebih rendah dan nilai parameter spesifik lebih tinggi.

Reconstructed Human Epidermis (RHE) is an artificial epidermis made in such a way that it resembles human skin, and can be used for the identification of irritant chemicals, especially for cosmetic and topical medicinal products. Currently the new RHE is produced by European and American countries, whose skin physiology is very different from Indonesia. Based on this, the Center for Research on Drugs and Food, NADFC of Republic of Indonesia took the initiative to develop the reconstruction of keratinocyte, melanocytes and fibroblasts cells into RHE adapted to the anatomical and physiological functions of the skin of Indonesians. RHE is made from an epidermal layer composed of keratinocyte and melanocytes cells that are reconstructed with a dermis layer composed of fibroblast and collagen cells. Keratinocyte, melanocytes and fibroblasts cells are cultured on suitable mediums by adding a suitable growth medium. To find out that RHE has been successfully reconstructed, measured percentage of cell life, made histology preparation to see the existence of cell nucleus, and conducted Immunohistochemical examination to see existence of integration (bond) between antigen. From the research results can be seen that keratinocyte cells grown on culture medium Keratinocyte SFM (IX) with rEGF supplements; melanocyte cells grown on Melanocyte 254 culture medium with HMGS supplementation; and fibroblast cells grown on Fibroblast M 106 culture medium with LSGS supplementation. The percentage of epidermal cell life grew well in the planting of 10 � 104 cells /mL keratinocytes and 0.25 � 104 cells /mL of melanocyte cells and survived until the 11th day with live cell percentage of 93.45%. In making preparation for histology with HE staining, there is a cell life in RHE tissue. Used Immunohistochemical (IHC) examination using cytokeratin 10 antibody marker to view physiological function of epidermal tissue.

Insektisida nabati dapat digunakan untuk mengendalikan dan mencegah penyakit yang disebabkan oleh nyamuk. Salah satu alternatif bahan insektisida nabati adalah daun zodia (Evodia suaveolens Scheff.) yang telah dikenal sebagai tanaman pengusir nyamuk. Penelitian ini bertujuan untuk mengetahui efek larvasida dan nilai LC50 dari ekstrak daun zodia terhadap larva nyamuk Aedes albopictus dan Culex spp. Penelitian ini menggunakan ekstrak nheksan daun zodia dengan variasi konsentrasi 10 ppm; 31,62 ppm; 99,98 ppm; 316,13 ppm; 1000 ppm dan kontrol. Metode yang digunakan adalah metode bioassay yang telah distandarisasi oleh WHO (2005). Data hasil penelitian dianalisis menggunakan uji probit. Hasil penelitian menunjukkan bahwa kematian 50% larva nyamuk Aedes albopictus dan Culex spp. (LC50-24 jam) adalah 106,036 ppm dan 116,9110 ppm. Berdasarkan hasil uji probit nilai LC50 masuk ke dalam rentang 100-149 ppm maka dapat disimpulkan bahwa ekstrak n-heksan daun zodia termasuk ke dalam golongan larvasida dengan tingkat toksisitas moderat tinggi.

BACKGROUND Memory is a vital function of the brain. Aerobic exercise has a positive effect on memory’s function, but the appropriate combination of intensity and duration of aerobic exercise is still unknown. This study was aimed to investigate the effect of optimum combinations of intensity and duration of aerobic exercise on spatial memory function.